Journal: Frontiers in Immunology
Article Title: Single-cell atlas of the tumor immune microenvironment across syngeneic murine models
doi: 10.3389/fimmu.2025.1676581
Figure Lengend Snippet: High-resolution profiling of TIICs across murine syngeneic tumor models. (A) Schematic representation of the experimental workflow for isolating and processing Cd45+ immune cells from 10 murine tumor models: breast mammary carcinoma (4T1, EMT6, MMTV-PyMT), colon carcinoma (CT26.WT, MC38), glioma (GL261), renal adenocarcinoma (Renca), lung carcinoma (LL2), melanoma (B16F10), and pancreatic adenocarcinoma (Pan02), with three biological replicates per model. (B) UMAP visualization of CCA integrated data depicting 7 major immune cell populations. (C) Expression patterns of canonical marker genes utilized to identify lymphoid (T cells, NK cells, B cells) and myeloid (DCs, Mo/Mφs, neutrophils, mast cells) lineages. (D) Percentages of major immune cell types in TIICs for each model.
Article Snippet: LL2 , ATCC (CRL-1642) , , DMEM (Gibco, Cat# 11965092) supplemented with 10% (v/v) fetal bovine serum (Gibco, Cat# 10099-141) and 100 ug/ml of penicillin and streptomycin (Gibco, Cat# 15140-122).
Techniques: Expressing, Marker